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transit r insect transfection reagent  (Mirus Bio)


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    Structured Review

    Mirus Bio transit r insect transfection reagent
    Transit R Insect Transfection Reagent, supplied by Mirus Bio, used in various techniques. Bioz Stars score: 96/100, based on 282 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/transit+r+insect+transfection+reagent/TransIT+-Insect/pmc13001065-77-8-11
    Average 96 stars, based on 282 article reviews
    transit r insect transfection reagent - by Bioz Stars, 2026-11
    96/100 stars

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    Related Articles

    Transfection:

    Article Title: Bombyx mori nucleopolyhedrovirus LEF-2 disrupts the cell cycle in the G2/M phase by triggering a host cell DNA damage response.
    Article Snippet: Associate Editor: Haobo Jiang Abstract It is a common strategy for viruses to block the host cell cycle to favour their DNA replication.. Baculovirus, being a double-stranded DNA virus, can arrest the cell cycle in the G2/M phase to facilitate its replication.. However, the key viral genes and mechanisms crucial for inducing cell cycle arrest remain poorly understood.

    Article Title: Bombyx mori Jun gene is involved in endoreplication of silk gland cells by regulating S-phase cell cycle genes.
    Article Snippet: .. Transient transfection and quantitative real-time polymerase chain reaction(qRT-PCR) Plasmids were transfected into BmN-SWU1 cells using the TransIT(R)-Insect Transfection Reagent (Mirus, Beijing, China) for 48 h. Total RNA was isolated using an RNA extraction kit (Omega Bio-Tek, Norcross, GA, USA), and cDNA was synthesized using a reverse transcription kit (Takara, Dalian, China). .. Quantitative real-time PCR (qRT-PCR) was performed using Novostart® SYBR qPCR Supermix Plus (Novo-protein, Shanghai, China) and the Real-time PCR System (Bio-Rad, Berkeley, CA, USA).

    Article Title: Bombyx mori Jun gene is involved in endoreplication of silk gland cells by regulating S-phase cell cycle genes
    Article Snippet: .. Plasmids were transfected into BmN-SWU1 cells using the TransIT(R)-Insect Transfection Reagent (Mirus, Beijing, China) for 48 h. Total RNA was isolated using an RNA extraction kit (Omega Bio-Tek, Norcross, GA, USA), and cDNA was synthesized using a reverse transcription kit (Takara, Dalian, China). .. Quantitative real-time PCR (qRT-PCR) was performed using Novostart SYBR qPCR Supermix Plus (Novo-protein, Shanghai, China) and the Real-time PCR System (Bio-Rad, Berkeley, CA, USA).

    Isolation:

    Article Title: Bombyx mori Jun gene is involved in endoreplication of silk gland cells by regulating S-phase cell cycle genes.
    Article Snippet: .. Transient transfection and quantitative real-time polymerase chain reaction(qRT-PCR) Plasmids were transfected into BmN-SWU1 cells using the TransIT(R)-Insect Transfection Reagent (Mirus, Beijing, China) for 48 h. Total RNA was isolated using an RNA extraction kit (Omega Bio-Tek, Norcross, GA, USA), and cDNA was synthesized using a reverse transcription kit (Takara, Dalian, China). .. Quantitative real-time PCR (qRT-PCR) was performed using Novostart® SYBR qPCR Supermix Plus (Novo-protein, Shanghai, China) and the Real-time PCR System (Bio-Rad, Berkeley, CA, USA).

    Article Title: Bombyx mori Jun gene is involved in endoreplication of silk gland cells by regulating S-phase cell cycle genes
    Article Snippet: .. Plasmids were transfected into BmN-SWU1 cells using the TransIT(R)-Insect Transfection Reagent (Mirus, Beijing, China) for 48 h. Total RNA was isolated using an RNA extraction kit (Omega Bio-Tek, Norcross, GA, USA), and cDNA was synthesized using a reverse transcription kit (Takara, Dalian, China). .. Quantitative real-time PCR (qRT-PCR) was performed using Novostart SYBR qPCR Supermix Plus (Novo-protein, Shanghai, China) and the Real-time PCR System (Bio-Rad, Berkeley, CA, USA).

    RNA Extraction:

    Article Title: Bombyx mori Jun gene is involved in endoreplication of silk gland cells by regulating S-phase cell cycle genes.
    Article Snippet: .. Transient transfection and quantitative real-time polymerase chain reaction(qRT-PCR) Plasmids were transfected into BmN-SWU1 cells using the TransIT(R)-Insect Transfection Reagent (Mirus, Beijing, China) for 48 h. Total RNA was isolated using an RNA extraction kit (Omega Bio-Tek, Norcross, GA, USA), and cDNA was synthesized using a reverse transcription kit (Takara, Dalian, China). .. Quantitative real-time PCR (qRT-PCR) was performed using Novostart® SYBR qPCR Supermix Plus (Novo-protein, Shanghai, China) and the Real-time PCR System (Bio-Rad, Berkeley, CA, USA).

    Article Title: Bombyx mori Jun gene is involved in endoreplication of silk gland cells by regulating S-phase cell cycle genes
    Article Snippet: .. Plasmids were transfected into BmN-SWU1 cells using the TransIT(R)-Insect Transfection Reagent (Mirus, Beijing, China) for 48 h. Total RNA was isolated using an RNA extraction kit (Omega Bio-Tek, Norcross, GA, USA), and cDNA was synthesized using a reverse transcription kit (Takara, Dalian, China). .. Quantitative real-time PCR (qRT-PCR) was performed using Novostart SYBR qPCR Supermix Plus (Novo-protein, Shanghai, China) and the Real-time PCR System (Bio-Rad, Berkeley, CA, USA).

    Synthesized:

    Article Title: Bombyx mori Jun gene is involved in endoreplication of silk gland cells by regulating S-phase cell cycle genes.
    Article Snippet: .. Transient transfection and quantitative real-time polymerase chain reaction(qRT-PCR) Plasmids were transfected into BmN-SWU1 cells using the TransIT(R)-Insect Transfection Reagent (Mirus, Beijing, China) for 48 h. Total RNA was isolated using an RNA extraction kit (Omega Bio-Tek, Norcross, GA, USA), and cDNA was synthesized using a reverse transcription kit (Takara, Dalian, China). .. Quantitative real-time PCR (qRT-PCR) was performed using Novostart® SYBR qPCR Supermix Plus (Novo-protein, Shanghai, China) and the Real-time PCR System (Bio-Rad, Berkeley, CA, USA).

    Article Title: Bombyx mori Jun gene is involved in endoreplication of silk gland cells by regulating S-phase cell cycle genes
    Article Snippet: .. Plasmids were transfected into BmN-SWU1 cells using the TransIT(R)-Insect Transfection Reagent (Mirus, Beijing, China) for 48 h. Total RNA was isolated using an RNA extraction kit (Omega Bio-Tek, Norcross, GA, USA), and cDNA was synthesized using a reverse transcription kit (Takara, Dalian, China). .. Quantitative real-time PCR (qRT-PCR) was performed using Novostart SYBR qPCR Supermix Plus (Novo-protein, Shanghai, China) and the Real-time PCR System (Bio-Rad, Berkeley, CA, USA).

    Reverse Transcription:

    Article Title: Bombyx mori Jun gene is involved in endoreplication of silk gland cells by regulating S-phase cell cycle genes.
    Article Snippet: .. Transient transfection and quantitative real-time polymerase chain reaction(qRT-PCR) Plasmids were transfected into BmN-SWU1 cells using the TransIT(R)-Insect Transfection Reagent (Mirus, Beijing, China) for 48 h. Total RNA was isolated using an RNA extraction kit (Omega Bio-Tek, Norcross, GA, USA), and cDNA was synthesized using a reverse transcription kit (Takara, Dalian, China). .. Quantitative real-time PCR (qRT-PCR) was performed using Novostart® SYBR qPCR Supermix Plus (Novo-protein, Shanghai, China) and the Real-time PCR System (Bio-Rad, Berkeley, CA, USA).

    Article Title: Bombyx mori Jun gene is involved in endoreplication of silk gland cells by regulating S-phase cell cycle genes
    Article Snippet: .. Plasmids were transfected into BmN-SWU1 cells using the TransIT(R)-Insect Transfection Reagent (Mirus, Beijing, China) for 48 h. Total RNA was isolated using an RNA extraction kit (Omega Bio-Tek, Norcross, GA, USA), and cDNA was synthesized using a reverse transcription kit (Takara, Dalian, China). .. Quantitative real-time PCR (qRT-PCR) was performed using Novostart SYBR qPCR Supermix Plus (Novo-protein, Shanghai, China) and the Real-time PCR System (Bio-Rad, Berkeley, CA, USA).



    Similar Products

    transit r insect transfection reagent  (Mirus Bio)
    96
    Mirus Bio transit r insect transfection reagent
    Transit R Insect Transfection Reagent, supplied by Mirus Bio, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/transit+r+insect+transfection+reagent/TransIT+-Insect/pmc13001065-77-8-11
    Average 96 stars, based on 1 article reviews
    transit r insect transfection reagent - by Bioz Stars, 2026-11
    96/100 stars
    		  Buy from Supplier
    trans it r insect transfection reagent  (Mirus Bio)
    96
    Mirus Bio trans it r insect transfection reagent
    BmNPV IAP1 interacts with BmCDK1. ( A ) BmCDK1 was overexpressed by recombinant BmNPV (vBm- BmCDK1 ). BmN-SWU1 cells were infected with 1 MOI of vBm- BmCDK1 . Co-immunoprecipitation (Co-IP) was performed 48 h p.i. Marker: protein molecular weight marker; Input: input cell lysates; IgG: IP with mouse IgG; Co-IP: IP with α-Flag monoclonal antibody. IgG and Co-IP bands of three independent experiments were combined and sent for LC–MS/MS analysis. IgG groups were applied as the negative control. ( B ) Candidate viral proteins interacting with BmCDK1 by LC–MS/MS. Blue box indicates the target protein for the subsequent investigations. ( C ) Co-IP of BmCDK1 and BmNPV IAP1. pIZ-BmNPV iap1 -HA was transfected into BmN-SWU1 cells, and cells were infected with 1 MOI of vBm- BmCDK1 48 h post <t>transfection.</t> Co-IP was analyzed with Western blotting 48 h p.i.
    Trans It R Insect Transfection Reagent, supplied by Mirus Bio, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/transit+r+insect+transfection+reagent/TransIT+-Insect/pmc08708760-73-7-12
    Average 96 stars, based on 1 article reviews
    trans it r insect transfection reagent - by Bioz Stars, 2026-11
    96/100 stars
    		  Buy from Supplier

    Image Search Results


    BmNPV IAP1 interacts with BmCDK1. ( A ) BmCDK1 was overexpressed by recombinant BmNPV (vBm- BmCDK1 ). BmN-SWU1 cells were infected with 1 MOI of vBm- BmCDK1 . Co-immunoprecipitation (Co-IP) was performed 48 h p.i. Marker: protein molecular weight marker; Input: input cell lysates; IgG: IP with mouse IgG; Co-IP: IP with α-Flag monoclonal antibody. IgG and Co-IP bands of three independent experiments were combined and sent for LC–MS/MS analysis. IgG groups were applied as the negative control. ( B ) Candidate viral proteins interacting with BmCDK1 by LC–MS/MS. Blue box indicates the target protein for the subsequent investigations. ( C ) Co-IP of BmCDK1 and BmNPV IAP1. pIZ-BmNPV iap1 -HA was transfected into BmN-SWU1 cells, and cells were infected with 1 MOI of vBm- BmCDK1 48 h post transfection. Co-IP was analyzed with Western blotting 48 h p.i.

    Journal: Insects

    Article Title: Bombyx mori Nucleopolyhedrovirus (BmNPV) Induces G2/M Arrest to Promote Viral Multiplication by Depleting BmCDK1

    doi: 10.3390/insects12121098

    Figure Lengend Snippet: BmNPV IAP1 interacts with BmCDK1. ( A ) BmCDK1 was overexpressed by recombinant BmNPV (vBm- BmCDK1 ). BmN-SWU1 cells were infected with 1 MOI of vBm- BmCDK1 . Co-immunoprecipitation (Co-IP) was performed 48 h p.i. Marker: protein molecular weight marker; Input: input cell lysates; IgG: IP with mouse IgG; Co-IP: IP with α-Flag monoclonal antibody. IgG and Co-IP bands of three independent experiments were combined and sent for LC–MS/MS analysis. IgG groups were applied as the negative control. ( B ) Candidate viral proteins interacting with BmCDK1 by LC–MS/MS. Blue box indicates the target protein for the subsequent investigations. ( C ) Co-IP of BmCDK1 and BmNPV IAP1. pIZ-BmNPV iap1 -HA was transfected into BmN-SWU1 cells, and cells were infected with 1 MOI of vBm- BmCDK1 48 h post transfection. Co-IP was analyzed with Western blotting 48 h p.i.

    Article Snippet: Transfection of BmN-SWU1 cells was performed with Trans IT (R)-Insect Transfection Reagent (Mirus, MIR6106).

    Techniques: Recombinant, Infection, Immunoprecipitation, Co-Immunoprecipitation Assay, Marker, Molecular Weight, Liquid Chromatography with Mass Spectroscopy, Negative Control, Transfection, Western Blot

    BmNPV iap1 regulates the cell cycle via BmCDK1 . ( A , B ) Comparison of cell cycle distribution in pIZ- eGFP and pIZ-BmNPV iap1 - eGFP groups. The pIZ- eGFP group was used as a negative control. The data were analyzed as mean ± SD from three independent experiments. ( C ) The amount of BmCDK1 was analyzed after co-transfecting with pIZ- BmCDK1 -Flag, pIZ-BmNPV iap1 -HA, pIZ- BmCDK1 -Flag, and pIZ-V5/His, successively. Tubulin was adopted as the loading control. ( D , E ) 48 h post transfection with control-KO and BmNPV iap1 -KO, cells were infected with BmNPV. The percentage of G2/M phase cells 24 h p.i. were presented as mean ± SD from three independent experiments. ( F ) The amount of BmCDK1 protein were detected in no-infected and 24 h p.i. cells using Western blotting. Tubulin was adopted as the loading control. The protein band density ratios of BmCDK1 were calculated by protein band density analysis using Image Lab. ( G ) Results shown with graphs representing the intensity of the BmCDK1/tubulin ratio. Statistical differences were analyzed with Student’s t -test (* p < 0.05 and ** p < 0.01).

    Journal: Insects

    Article Title: Bombyx mori Nucleopolyhedrovirus (BmNPV) Induces G2/M Arrest to Promote Viral Multiplication by Depleting BmCDK1

    doi: 10.3390/insects12121098

    Figure Lengend Snippet: BmNPV iap1 regulates the cell cycle via BmCDK1 . ( A , B ) Comparison of cell cycle distribution in pIZ- eGFP and pIZ-BmNPV iap1 - eGFP groups. The pIZ- eGFP group was used as a negative control. The data were analyzed as mean ± SD from three independent experiments. ( C ) The amount of BmCDK1 was analyzed after co-transfecting with pIZ- BmCDK1 -Flag, pIZ-BmNPV iap1 -HA, pIZ- BmCDK1 -Flag, and pIZ-V5/His, successively. Tubulin was adopted as the loading control. ( D , E ) 48 h post transfection with control-KO and BmNPV iap1 -KO, cells were infected with BmNPV. The percentage of G2/M phase cells 24 h p.i. were presented as mean ± SD from three independent experiments. ( F ) The amount of BmCDK1 protein were detected in no-infected and 24 h p.i. cells using Western blotting. Tubulin was adopted as the loading control. The protein band density ratios of BmCDK1 were calculated by protein band density analysis using Image Lab. ( G ) Results shown with graphs representing the intensity of the BmCDK1/tubulin ratio. Statistical differences were analyzed with Student’s t -test (* p < 0.05 and ** p < 0.01).

    Article Snippet: Transfection of BmN-SWU1 cells was performed with Trans IT (R)-Insect Transfection Reagent (Mirus, MIR6106).

    Techniques: Comparison, Negative Control, Control, Transfection, Infection, Western Blot